|
|
|
|
|
| Duan,Hai-long; He,Qi-yi; Zhou,Bin; Wang,Wen-wen; Li,Bo; Zhang,Ying-zheng; Deng,Qiu-ping; Zhang,Ying-feng; Yu,Xiao-dong. |
| Abstract Background Snakebite incidence in southwestern China is mainly attributed to one of the several venomous snakes found in the country, the white-lipped green pit viper Trimeresurus albolabris. Since antivenom produced from horses may cause numerous clinical side effects, the present study was conducted aiming to develop an alternative antivenom antibody (immunoglobulin Y - IgY) from leghorn chickens. Methods IgY in egg yolk from white leghorn chicken previously injected with T. albolabris venom was extracted by water, precipitated by ammonium sulfate and purified by affinity chromatographic system. IgY was identified by SDS-PAGE, ELISA and Western blot, and its neutralizing assay was conducted on mice. Results Chickens injected multiple times... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: IgY; Egg yolk; Snake venom; Trimeresurus albolabris; LD50. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100316 |
| |
|
| |
|
|
| Freire,Ronald Bastos; Freire,Márcia de Fátima Inácio; Berbara,Ricardo Louro. |
| O presente experimento descreve, pela primeira vez, a elaboração de um ensaio imunoenzimático (ELISA de captura) com anticorpos IgY para a detecção e quantificação de acetato de lupeol (LAc) em Vernonia scorpioides Lam. Pers (Asteraceae). Anticorpos anti-LAc, obtidos partir do soro das aves e gemas de seus ovos, após imunização com o conjugado acetato de lupeol-cBSA, foram concentrados em coluna contendo LAc-BSA acoplado a sepharose. A eficácia da metodologia de detecção imunológica foi de 97%, com índices de sensibilidade e especificidade de 99% e 95%, respectivamente com limites de detecção do ensaio entre 0,02µg.g-1 (inferior) e 10 µg.g-1 (superior). A robustez do método foi atestada pela sua elevada reprodutibilidade (entre 94,75% e 96,81%), e pelo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: IgY-ELISA; IgY; Vernonia scorpioides; Acetato de lupeol; Controle de qualidade vegetal. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000400016 |
| |
|
|
| Silva,Marco Aurélio Chiara; Silva,Miriele Caroline da; Pinheiro,João Waine; Castro-Goméz,Raul Jorge Hernan; Murakami,Alice Eiko; Loyola,Wagner; Venancio,Emerson José. |
| ABSTRACT: Advances in the fields of glycobiology and immunology have provided many insights into the role of carbohydrate-protein interactions in the immune system. Jacalin of Artocarpus integrifolia (JCA) and structural mannoprotein of Saccharomyces uvarum (MPS) are molecules with immunomodulatory properties. JCA is an IgA human lectin binding molecule that causes the mitogenic stimulation of immune cells, production of cytokines, chemotaxis, and activation of leukocytes. Studies on the immunomodulatory properties of JCA and MPS in mammals and fish suggest that they have an action on antibody production. The aim of this study was to investigate the possible action of JCA and MPS on the production of specific antibodies in laying hens. For this, laying... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artocarpus integrifolia; Chickens; IgY; Jacalin; Mannoprotein; Humoral immune response; Saccharomyces uvarum. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000400453 |
| |
|
|
| SOARES,DANIELA F.; PIZZOLANTE,CARLA C.; DUARTE,KEILA MARIA R.; MORAES,JOSÉ EVANDRO DE; BUDIÑO,FÁBIO E.L.; SOARES,WEBER V.B.; KAKIMOTO,SÉRGIO K.. |
| Abstract In the way to reduce costs in the poultry production, high densities of birds are adopted in the production systems. Such high densities can lead to an inappropriate environment, compromising the welfare and decreasing the animal performance. In this work we aimed to evaluate the effects of different densities on the productive yield, egg quality and immunological aspects of Japanese quails. It was used 816 Japanese quails, during egg production phase, in a random design, with four treatments (121.43 cm2/bird; 106.25 cm2/bird; 94.44 cm2/bird and 85.00 cm2/bird) with six repetitions each. Results indicate density did not interfere on egg quality and all treatments can be considered excellent, once the Haugh Unit was 88.03. Therefore, higher... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immunity; IgY; Egg quality; Haugh Unit. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703791 |
| |
|
|
|
